鸭骨骼肌早期发育过程中钙蛋白酶3（CAPN3）基因的表达及其与肌纤维性状的关联

doi:10.11843/j.issn.0366-6964.2014.03.007

Abstract

Abstract:

In the present study，expression of CAPN3 gene was quantified by RT-PCR in the breast muscle and leg muscle tissues on days 13(E13 d)，17(E17 d)，21(E21 d)，25(E25 d)，27(E27 d) of embryonic development，as well as at 7 days post-hatching (7 d PH) in Gaoyou and Jinding ducks with different growth rates.Meanwhile，the association between expression level of CAPN3 gene and myofiber traits was also analyzed. The results showed that the CAPN3 mRNA expression profile had a similar trend in both breeds and showed an extremely significant specific expression at different development stages，breeds and gender had no effect on its expression.In breast muscle，the expression level of CAPN3 mRNA was relatively low before E25 d and the lowest point appeared on E21 d，then increased significantly before hatching (E27 d) and 7 d PH.In leg muscle，the expression profile of CAPN3 showed a “wave” trend，the expression peaking appeared on E21 d.Meanwhile different linear correlations were found between CAPN3 gene mRNA expression and myofiber types，diameter，cross-sectional area and density in duck.In Jinding ducks，CAPN3 gene mRNA expression was significantly correlated with myofiber diameter，cross-sectional area and density.These results suggest that CAPN3 may have potential functions in controlling muscle fiber phenotype and development during embryonic and early post-hatching development in ducks.

CLC Number:

S834
S813.3

ZHU Wen-qi，XU Wen-juan，SHU Jing-ting，CHEN Wen-feng，SHAN Yan-ju，LIU Hong-xiang，HU Yan，LI Hui-fang，WANG Jin-yu. Expression of CAPN3 Gene in Skeletal Muscles and Its Association with Myofiber Traits during Embryonic and Early Post-hatching Development in Ducks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 385-390.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.03.007

https://www.xmsyxb.com/EN/Y2014/V45/I3/385

References

［1］HOPKINS D L，THOMPSON J M.Inhibition of protease activity Part l.The effect on tenderness and indicators of proteolysis in ovine muscle ［J］.Meat Sci，2001，59：175-185.
［2］CROALL D E，DEMARTINO G N.Calcium- activated neutral protease (calpain) system：structure，functure，and regulation ［J］. Physiol Rev, 1991，71(3)：813-847.
［3］SORIMACH I H，IMAJOH-OHMI S，EMORI Y，et al.Molecular cloning of a novel mammalian calciumdependent protease distinct from both m- and Ltypes.Specific expression of the mRNA in skeletal muscle ［J］.J Biol Chem，1989，264：20106-20111.
［4］LABEIT S，KOLMERER B，LINKE W A.The giant protein titin：Emerging roles in physiology and pathophysiology ［J］.Circ Res，1997，80(2)：284-290.
［5］SHOWALTER C J，ENGEL A G.Acute quadriplegic myopathy：analysis of myosin isoforms and evidence for calpain-mediated proteolysis ［J］.Muscle Nerve，1997，20(3)：316-322.
［6］ILIAN M A，MORTON J D，KENTM P，et al.Intermuscular variation in tenderness：Association with the ubiquitous and muscle specific calpain ［J］.J Anim Sci，200la，79(1)：122-132.
［7］ILIAN M A，MORTON J D，BEKHIT A E，et al.Effect of preslaughter feed withdrawal period on longissimus tenderness and the expression of calpains in the ovine ［J］.J Agric Food Chem，200lb，49(4)：1990-1998.
［8］OJIMA K，ONO Y，HATA S，et al.Possible functions of p94 in cormectin-mediated signaling pathways in skeletal muscle cells［J］.J Muscle Res Cell Motility，2005，26(6-8)：409-417.
［9］MURPHY R M，VERBURG E，LAMB G D.Ca²⁺ activation of diffusible and bound pools of mu-calpain in rat skeletal muscle［J］.J Physiol，2006，576(2)：595-612.
［10］张增荣.鸡CAPN1、CAPN2、CAPN3和CAST基因的克隆、表达及其在肉质中的遗传效应分析［D］.雅安：四川农业大学，2009.
［11］ZHANG Z R，ZHU Q，YAO Y G，et al.Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken ［J］.Mol Biol Rep，2012，39：3517-3521.
［12］FELíCIO A M，BOSCHIERO C，BALIEIRO J C，et al.Identification and association of polymorphisms in CAPN1 and CAPN3 candidate genes related to performance and meat quality traits in chickens ［J］.Mol Biol Rep，2013，12(1)：472-482.
［13］GUTH L，SAMAHA F J.Procedure for the histochemical demonstration of actomyosin ATPase ［J］.Exp Neurol，1970，28(2)：365-367.
［14］PICARD B，LEFAUCHEUR L，BERRI C，et al.Muscle fibre ontogenesis in farm animal species［J］.Repord Nutr Dev，2002，42(5)：415-431.
［15］WIGMORE P M，DUNGLISON G F.The generation of fiber diversity during myogenesis［J］.Int J Dev Biol，1998，42：117-125.
［16］CONDON K，SILBERSTEIN L，BLAU H M，et al.Development of muscle fiber types in the prenatal rat hindlimb［J］.Dev Biol，1990，138(2)：256-274.

[1]	GUO Jia-jia，DAI Zi-chun，LU Chen，ZHANG Tian，SHI Zhen-dan，ZHAO Wei，MA Wei-ming，YING Shi-jia. Expression of Toll-like Receptors in Duck Intestinal Tract at the Peak and End of Lay [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2012-2019.
[2]	CHEN Fang，ZHANG Hao，LU Li-zhi. Molecular Cloning，Expression Pattern and Function of Duck I-FABP Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 1041-1048.
[3]	WANG Hao-han, LIU He-he, SUN Ling-li, ZHANG Rong-ping, LI Xin-xin, YANG Chao, LI Liang, WANG Ji-wen. Molecule Cloning and Developmental Expression of Mef2bnb Gene in Muscle Tissues from Duck [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(1): 49-56.

Expression of CAPN3 Gene in Skeletal Muscles and Its Association with Myofiber Traits during Embryonic and Early Post-hatching Development in Ducks

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments